| WEEK | DATE | OPPONENT | TIME (ET) | Location |
| Pre | Sun, Aug 9th, 2009 | at Tennessee | 08:00 PM | Hall-of-Fame Game - Canton, OH |
| Pre | Sat, Aug 15th, 2009 | Chicago | 07:00 PM | Ralph Wilson Stadium |
| Pre | Sat, Aug 22nd, 2009 | at Green_Bay | 08:00 PM | Lambeau Field |
| Pre | Sat, Aug 29th, 2009 | at Pittsburgh | 07:30 PM | Heinz Field |
| Pre | Thu, Sep 3rd, 2009 | Detroit | 06:30 PM | Ralph Wilson Stadium |
| 1 | Mon, Sep 14th, 2009 | at New_England | 07:00 PM | Gillette Stadium |
| 2 | Sun, Sep 20th, 2009 | Tampa_Bay | 04:05 PM | Ralph Wilson Stadium |
| 3 | Sun, Sep 27th, 2009 | New_Orleans | 04:05 PM | Ralph Wilson Stadium |
| 4 | Sun, Oct 4th, 2009 | at Miami | 04:05 PM | LandShark Stadium |
| 5 | Sun, Oct 11th, 2009 | Cleveland | 01:00 PM | Ralph Wilson Stadium |
| 6 | Sun, Oct 18th, 2009 | at NY_Jets | 04:15 PM | Meadowlands |
| 7 | Sun, Oct 25th, 2009 | at Carolina | 04:05 PM | Bank of America Stadium |
| 8 | Sun, Nov 1st, 2009 | Houston | 01:00 PM | Ralph Wilson Stadium |
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| 10 | Sun, Nov 15th, 2009 | at Tennessee | 01:00 PM | LP Field |
| 11 | Sun, Nov 22nd, 2009 | at Jacksonville | 01:00 PM | Jacksonville Municipal Stadium |
| 12 | Sun, Nov 29th, 2009 | Miami | 01:00 PM | Ralph Wilson Stadium |
| 13 | Thu, Dec 3rd, 2009 | NY_Jets | 08:20 PM | Rogers Centre - Toronto, Ontar |
| 14 | Sun, Dec 13th, 2009 | at Kansas_City | 01:00 PM | Arrowhead Stadium |
| 15 | Sun, Dec 20th, 2009 | New_England | 01:00 PM | Ralph Wilson Stadium |
| 16 | Sun, Dec 27th, 2009 | at Atlanta | 01:00 PM | Georgia Dome |
| 17 | Sun, Jan 3rd, 2010 | Indianapolis | 01:00 PM | Ralph Wilson Stadium |